Mathews made his T20 comeback for Sri Lanka in January after a three-year gap
Both teams were unchanged from the first of this five-match series, which England won by 24 runs at the Riverside on Friday.
Pakistan’s decision to stick with the same side meant there was still no place for all-rounder Abdul Razzaq who has been struggling with shoulder and back injuries.
Once again fast bowler Wahab Riaz, who will be interviewed by police next week in the course of their spot-fixing inquiries, was omitted.
Pakistan’s tour of Britain has been overshadowed by newspaper allegations of a betting scam that saw no-balls deliberately bowled in the fourth Test against England at Lord’s last month.
The claims, published in the News of the World, led to the suspension of Pakistan Test captain Salman Butt and bowlers Mohammad Aamer and Mohammad Asif by the International Cricket Council.
England: Andrew Strauss (capt), Steve Davies (wkt), Jonathan Trott, Paul Collingwood, Eoin Morgan, Ravi Bopara, Tim Bresnan, Michael Yardy, Graeme Swann, Stuart Broad, James Anderson
Pakistan: Kamran Akmal (wkt), Mohammad Hafeez, Asad Shafiq, Mohammad Yousuf, Fawad Alam, Umar Akmal, Shahid Afridi (capt), Umar Gul, Saeed Ajmal, Shoaib Akhtar, Mohammad Irfan
Umpires: Nigel Llong (ENG) and Billy Doctrove (WIS)
TV umpire: Ian Gould (ENG)
Match referee: Jeff Crowe (NZL)
Mathews made his T20 comeback for Sri Lanka in January after a three-year gap
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