The England international being benched means Manuel Akanji starts on the right of a three-man defence
Australian Open organisers announced Azarenka's withdrawal in a social media post on Monday, a week before the tournament begins.
"It's unfortunate that (Azarenka) is unable to travel to Australia this year," organisers posted on Twitter, quoting tournament director Craig Tiley. The Australian Open, "is her favourite tournament and she's looking forward to returning to Melbourne next year."
Azarenka, who won the Australian Open in 2012 and 2013, has been involved in a custody dispute involving her son Leo, who was born last Dec. 19.
She hasn't competed anywhere since Wimbledon, where she lost to Simona Halep in the fourth round on July 10.
Former No. 1-ranked Azarenka played only six matches in 2017 and her year-ending ranking plummeted to No. 208.
Australian Open organisers last month granted her a wildcard entry, with Tiley saying at the time, "Vika's current situation is obviously very difficult for her and we have reached out to offer any support we can."
Azarenka had initially been scheduled to make a return to competitive tennis in New Zealand last week, but withdrew from that tournament as well.
The Australian Open starts next Monday at Melbourne Park. Defending champion Serena Williams has already withdrawn, saying her game wasn't back to a level where she believed she could contend for the title just four months after giving birth to her first child.
The England international being benched means Manuel Akanji starts on the right of a three-man defence
Prime Minister Shahbaz Sharif expresses grief over the loss of life from the storm
The regulation was removed from the ICC Playing Conditions at the start of June, with the new laws coming into play for the first time when England played Ireland at Lord's a week ago
The doyens of Indian cinema, who starred in
A highly competitive field of nine horses led by 2-year-old champion Forte and Preakness winner National Treasure set to run in the 2,400 metre test
Sheikh Mohamed will hold talks with President Erdogan with a focus on further enhancing economic cooperation bet ween the countries and supporting regional stability
He has been accused of killing her, however he claims that she died by suicide
Swiatek became the first woman to successfully defend the title in Paris in 16 years