The Jamaican currently holds world records in the 100m, 200m and 4x100m with times of 9.58s, 19.19s and 36.84s respectively
According to Variety, the former lovebirds were joined by other celebrities like Jimmy Kimmel, John Legend, Julia Roberts, Shia LaBeouf, Morgan Freeman, Henry Golding and, Ray Liotta.
The one-hour-long fundraiser event streamed on the Facebook page for Penn's non-profit CORE (Community Organized Relief Effort) and LiveXLive as well as the criminal justice reform group Reform Alliance.
The show was hosted by Dane Cook, while Freeman did the narration part of the unrehearsed read.
A majority of the attention of the star-studded event went on to Pitt and Aniston who were seen together on a screen after 15 years of their divorce. The two were seen reading out raunchy scene from the teen drama.
While Pitt voiced Brad Hamilton, which was originally played by Judge Reinhold in the film, Aniston played the character of Linda Barrett.
The show was originally scheduled to stream a month ago but was delayed due to "technical difficulties."
Brad and Jennifer made headlines earlier this year with their backstage reunion at the SAG award ceremony.
The Jamaican currently holds world records in the 100m, 200m and 4x100m with times of 9.58s, 19.19s and 36.84s respectively
Total property income posted year-on-year growth of 10% to $74m, highest value ever
All official donation channels however remain open through the outlets announced by government agencies
The Starlight Park is a four-building freehold community in Meydan
The 22-time major champion admits his farewell tour hasn't been as enjoyable as he would have hoped
Eating out, poor hand hygiene and erratic weather may be among causes, say medics
The goal-scoring striker is suffering from a muscle injury sustained during the Champions League quarter-final loss to Real Madrid
Lieutenant General Abdullah Khalifa Al Marri, Commander-in-Chief of Dubai Police, made the announcement on Wednesday